SKU: 33446917719

UA-Glo® cAMP Reagent

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Description

UA-Glo® cAMP ReagentProduct Specification Stability & Storage Dry ice transportation. Store protected from light at 80C. Upon initial use, prepare as UA Glo cAMP reagent stock solution, then aliquot as needed and store protected from light at 80C, avoiding repeated freeze thaw cycles. Background The UA Glo cAMP reagent is suitable for measuring changes in cAMP concentration in live cells using the Promega GloSensor cAMP assay system, with detection performance comparable

Product Specification


Stability & Storage

Dry ice transportation. Store protected from light at -80°C. Upon initial use, prepare as UA-Glo® cAMP reagent stock solution, then aliquot as needed and store protected from light at -80°C, avoiding repeated freeze-thaw cycles.

Background

The UA-Glo® cAMP reagent is suitable for measuring changes in cAMP concentration in live cells using the Promega GloSensor™ cAMP assay system, with detection performance comparable to that of the Promega GloSensor™ cAMP Reagent (Cat E1290, E1291).

Components

The components and specifications of UA-Glo® cAMP reagent are as follows. The detectable number of wells in 96-/384-well plates is calculated based on the standard experimental conditions where 100/20 μL of balanced medium is added per well in 96-/384-well plates, respectively.

cAMP Reagent

HEPES Buffer (10mM, pH7.5)

Detectable 96-Well Plate Wells

Detectable 384-Well Plate Wells

25 mg

1 mL

2,000

250 mg

10 mL

20,000


Protocol

According to the Promega GloSensor™ cAMP assay system manual (TM076), design and perform relevant experiments. Below are the experimental steps for cAMP detection in adherent cells using UA-Glo® cAMP reagent.

1)Prepare UA-Glo® cAMP reagent stock solution: Add 25mg UA-Glo® cAMP reagent (L7101) to 817μL of 10mM HEPES buffer (pH7.5), or add 250mg UA-Glo® cAMP reagent (L7102) to 8.17mL HEPES buffer (pH7.5). Mix thoroughly. Aliquot as needed and store protected from light at -80, avoiding repeated freeze-thaw cycles. This cAMP reagent stock solution can be diluted 50x as described in step 3 to prepare equilibration medium, or the concentration of cAMP reagent in the equilibration medium can be optimized for maximum signal-to-background difference.

2)Prepare equilibration medium aseptically as needed:

88% CO2-independent medium (e.g., Invitrogen Cat.#18045 )

10% fetal bovine serum

2% UA-Glo® cAMP reagent stock solution

3)Remove the 96-well experimental cell plate (e.g., cells expressing Promega GloSensor™ or transiently transfected cells), carefully aspirate the medium from the wells, and gently add 100μL of the equilibration medium prepared in step 2. Incubate at room temperature (22-25) for 2hr. The incubation time and temperature can be optimized based on when background signals stabilize. Ensure consistent temperature across the plate during detection.

4)Add test compounds and appropriate controls according to the experimental design. For example, use 10μM forskolin as a positive control and the solvent vehicle as a negative control.

5)Measure luminescence signals using a luminometer or multifunctional plate reader 15-30min after compound addition. Refer to section 3.A of the Promega GloSensor™ cAMP assay system manual (TM076) for specific timing or optimize based on the experiment.

Guidelines

1) Different batches are not recommended for mixed use 2) It is not recommended to change the dosage of detection reagents without strict validation 3) For research use only
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SKU: 33446917719

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Bee
Cuba, US
★★★★★ 5
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Color: (001) Black / Black / Reflective, Size: Large
Absolutely love these gloves and they are perfect for a fall morning run or as an extra layer inside a mitten I have noticed under armour gloves seem to run slightly small but the large fit ne perfect and id consider my hand a little on the smaller side these fit perfectly snug
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Reviewed in the United States on December 3, 2025
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Donald
Phoenix, US
★★★★★ 5
work gloves
Color: (001) Black / Black / Reflective, Size: Large
great gloves... love em
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Reviewed in the United States on May 23, 2025
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Anna
Birmingham, US
★★★★★ 3
Lightweight, good touchscreen functionality, poor sewing quality
Color: Black (002)/Black, Size: Small, Color: Black (002)/Black, Size: Small
I really liked these gloves. They are thin, but provided a fair amount of warmth. The touch screen worked great. I also thought the little pocket was a great addition. The downside is the second time I wore them, the seam on the thumb split. Not sure if it was a defect in just this pair or a recurring thing as my size was sold out when I attempted to exchange them.
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Reviewed in the United States on November 22, 2021
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Martha A. Wells
Cuba, US
★★★★★ 5
Quality Gloves
Color: Black (001)/Jet Gray, Size: Large
May run a little small, but a quality product
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Reviewed in the United States on January 1, 2026
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Lisa
Los Angeles, US
★★★★★ 5
Nice gloves
Color: Black (001)/Jet Gray, Size: Medium
These are my second pair I lost one from my first pair and was happy I could still buy these they are so comfortable and warm for cold weather but not super cold Fit was perfect
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Reviewed in the United States on January 13, 2026

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