SKU: 13492098703

Mouse OLFM4 (Olfactomedin 4) ELISA Kit

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Description

Mouse OLFM4 (Olfactomedin 4) ELISA KitThe Mouse OLFM4 (Olfactomedin 4) ELISA Kit measures OLFM4 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OLFM4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Mouse OLFM4 (Olfactomedin 4) ELISA Kit measures OLFM4 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OLFM4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with OLFM4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of OLFM4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 13492098703

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Ruth Euler
Lake Worth, US
★★★★★ 5
Big book of construction machines
Format: Hardcover
Book was a gift for a 3 year old. He loved it.
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Reviewed in the United States on June 16, 2023
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Amazon Customer
Lexington, US
★★★★★ 5
Great book
Format: Hardcover
My 4,5 loved it ! The fold out pages were a big hit !
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Reviewed in the United States on March 4, 2021
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Heather B.
Draper, US
★★★★★ 4
Adequate for our use.....
Format: Hardcover
The book arrived a week before expected and they were true to their evaluation that this was a used book in "acceptable condition". It was once a library book and has been well loved. Someone has taken contact paper to try to salvage the cover and binding. It looks rumply, but we were getting this for a 1 year old whose Dad is going to go to Heavy Equipment School for the next 4 years, so this will be a fine book for a little guy as the insides look fine.....I wouldn't have been happy if I'd wanted a new looking book, but the price was very reasonable. I think it was $3.99 for shipping and a penny for the book. SUCHA DEAL!
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Reviewed in the United States on April 20, 2016
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seven_scuba
Los Angeles, US
★★★★★ 5
Great Book!
Format: Hardcover
We bought this book a year ago for our then almost three-year-old. He loves it. As does his older sister (8), and brother (6) and his younger brother (almost 2). It has held up amazingly well considering the amount or use and abuse it gets. I love that it tells actual names and functions. More than once we've seen a machine while driving around town, then come home and looked it up. Well worth the money.
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Reviewed in the United States on October 9, 2011
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PrairieGirl
Pawtucket, US
★★★★★ 5
Great!
Format: Hardcover
This book came in time for my grandson's birthday. It is FANTASTIC! It is large, colorful and has gatefold pages (which I didn't realize). He loves it and we love reading it to him.
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Reviewed in the United States on October 2, 2015

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