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Description
Human ATG3 (Ubiquitin-like-conjugating Enzyme Atg3) ELISA KitThe Human (ATG3) Ubiquitin like conjugating Enzyme Atg3 ELISA Kit measures Ubiquitin Like Conjugating Enzyme ATG3 in samples. The plate has been pre coated with Human ATG3 antibody. ATG3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATG3 Antibody is added and binds to ATG3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ATG3 antibody. After incubation unbound
The Human (ATG3) Ubiquitin-like-conjugating Enzyme Atg3 ELISA Kit measures Ubiquitin-Like-Conjugating Enzyme ATG3 in samples. The plate has been pre-coated with Human ATG3 antibody. ATG3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATG3 Antibody is added and binds to ATG3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATG3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ATG3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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