Mouse EGF Receptor ELISA Kit

Mouse EGF Receptor ELISA KitThe Mouse EGF Receptor ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to EGFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to EGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells

The Mouse EGF Receptor ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.