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Description
Protein G magnetic microspheresProduct Name Protein G magnetic microspheres Product Overview Protein G magnetic beads are high performance affinity particles used in biomedical research, primarily for antibody purification and immunoprecipitation methods. They are typically made of superparamagnetic material and are coated with recombinant Protein G, a protein that specifically binds a wide range of mammalian IgGs. Technical Parameter Concentration: 30 mg mL Solvent: water
Product Name
Protein G magnetic microspheres
Product Overview
Protein G magnetic beads are high-performance affinity particles used in biomedical research, primarily for antibody purification and immunoprecipitation methods. They are typically made of superparamagnetic material and are coated with recombinant Protein G, a protein that specifically binds a wide range of mammalian IgGs.
Technical Parameter
Concentration: 30 mg/mL
Solvent: water
Diameter: ~1um
SPA load : 70-85ug/mg
Surface potential:~- 26 mV
Magnetic content: 35%-45%
Product Features
High capacity: high binding capacity, low sample volume per experiment .
Low non-specific binding: Stable pre-enclosed magnetic beads provide high purity products.
Flexibility: One bead contains both IgG binding domains of protein G, which is convenient to use.
Application Fields
Immunoprecipitation (IP) and Co-immunoprecipitation (Co-IP) assays: Protein G beads can be used to precipitate specific antibodies or their complexes from samples from a wide range of species, including mice, humans, rabbits, ewes, goats and sheep.
Antibody Purification: Particularly useful for purifying antibodies, including many IgG subclasses, from serum, cell culture supernatants or ascites.
Chemical cross-linking: antibodies can be immobilised onto magnetic beads using chemical cross-linkers to avoid IgG contamination in IP or Co-IP experiments, creating reusable immunoprecipitation beads.
Related Information
Sealed, avoid light, and keep at 4¡ãC Expiry date: Six months before unsealing.
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