Mouse TOP1 (Topoisomerase I) ELISA Kit

Mouse TOP1 (Topoisomerase I) ELISA KitThe Mouse TOP1 (Topoisomerase I) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse TOP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TOP1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate

The Mouse TOP1 (Topoisomerase I) ELISA immunoassay kit uses the Sandwich-ELISA  principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody  specific to Mouse TOP1. Standards or samples are added to the micro ELISA plate wells  and combined with the specific antibody. Then a biotinylated detection antibody specific  for Mouse TOP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added  successively to each micro plate well and incubated. Free components are washed away.  The substrate solution is added to each well. Only those wells that contain Mouse TOP1,  biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The  enzyme-substrate reaction is terminated by the addition of stop solution and the color  turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength  of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TOP1. You  can calculate the concentration of Mouse TOP1 in the samples by comparing the OD of the samples to the standard curve.