SKU: 52615289251

Rat NF-κB p105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit (Small Sample)

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Description

Rat NF-κB p105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit (Small Sample)The Rat NF B p105 (Nuclear factor NF kappa B p105 subunit) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat NF Bp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat NF Bp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After

The Rat NF-κB p105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 52615289251

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Format: Hardcover, Format: Hardcover
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Reviewed in the United States on December 6, 2025
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Reviewed in the United States on September 18, 2024

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