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Description
Human SRD5A1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1) ELISA KitThe Human (SRD5A1) 3 oxo 5 alpha steroid 4 dehydrogenase 1 ELISA Kit measures 3 oxo 5 alpha steroid 4 dehydrogenase 1 in samples. The plate has been pre coated with Human SRD5A1 antibody. SRD5A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SRD5A1 Antibody is added and binds to SRD5A1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SRD5A1 antibody. After incubation
The Human (SRD5A1) 3-oxo-5-alpha-steroid 4-dehydrogenase 1 ELISA Kit measures 3-oxo-5-alpha-steroid 4-dehydrogenase 1 in samples. The plate has been pre-coated with Human SRD5A1 antibody. SRD5A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SRD5A1 Antibody is added and binds to SRD5A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SRD5A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SRD5A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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