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Ships within 48 hours · Estimated delivery Jul 27 - Aug 1
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Description
Human TPO-IgE (Anti-Thyroid peroxidase Immunoglobulin E) ELISA KitThe Human TPO IgE (Anti Thyroid peroxidase Immunoglobulin E) ELISA Kit measures anti TPO IgE in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Biotinylated antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After
The Human TPO-IgE (Anti-Thyroid peroxidase Immunoglobulin E) ELISA Kit measures anti-TPO-IgE in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Biotinylated antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with anti-TPO-IgE bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-TPO-IgE in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
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