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Description
Human EN2 (Homeobox Protein Engrailed-2) ELISA KitThe Human (EN2) Homeobox Protein Engrailed 2 ELISA Kit measures Homeobox protein engrailed 2 in samples. The plate has been pre coated with Human EN2 antibody. EN2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human EN2 Antibody is added and binds to EN2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated EN2 antibody. After incubation unbound Streptavidin HRP is washed away
The Human (EN2) Homeobox Protein Engrailed-2 ELISA Kit measures Homeobox protein engrailed-2 in samples. The plate has been pre-coated with Human EN2 antibody. EN2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human EN2 Antibody is added and binds to EN2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated EN2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human EN2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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