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Description
Human PUM1 (Pumilio Homolog 1) ELISA KitThe Human (PUM1) Pumilio Homolog 1 ELISA Kit measures Pumilio Homolog 1 in samples. The plate has been pre coated with Human PUM1 antibody. PUM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PUM1 Antibody is added and binds to PUM1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PUM1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
The Human (PUM1) Pumilio Homolog 1 ELISA Kit measures Pumilio Homolog 1 in samples. The plate has been pre-coated with Human PUM1 antibody. PUM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PUM1 Antibody is added and binds to PUM1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PUM1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PUM1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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