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Description
Mouse IL-10 Serum & Plasma & Cell culture extracts OneStep ELISA KitProduct Specification Antigen IL 10 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Ms Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 2. 1% Inter assay: 4. 4% Sample type Serum; Citrate plasma; Cell culture extracts Assay type Sandwich (quantitative) Sensitivity 1. 864 pg mL Range 12. 5 pg mL 800 pg mL Recovery Serum: 89% Citrate plasma:
Product Specification
| Antigen | IL-10 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.1%； Inter-assay: 4.4% |
| Sample type | Serum; Citrate plasma; Cell culture extracts |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.864 pg/mL |
| Range | 12.5 pg/mL – 800 pg/mL |
| Recovery | Serum: 89% Citrate plasma: 115% Cell culture extracts: 112% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse Interleukin-10 (IL-10) is a potent, pleiotropic cytokine that plays a critical role as a central regulator of immune responses; it is a homodimeric protein produced by a wide variety of immune cells, including macrophages, dendritic cells, and T and B lymphocytes, and its primary biological function is to limit and ultimately terminate inflammatory responses by inhibiting the expression of pro-inflammatory cytokines (such as TNF-α, IL-1, and IL-6) and chemokines from activated macrophages and dendritic cells, while also downregulating major histocompatibility complex (MHC) class II and co-stimulatory molecules to suppress antigen presentation and T-cell activation. By controlling the magnitude and duration of inflammation, Mouse IL-10 is essential for protecting the host from immunopathology during infections and autoimmune reactions, though its potent anti-inflammatory activity can also be exploited by pathogens to evade the immune system; due to its central role in immune homeostasis, the IL-10 gene knockout mouse is a valuable model for studying inflammatory bowel disease (IBD) and other chronic inflammatory conditions.
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