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Description
c-Raf Recombinant Rabbit mAb (S-2718-61)Product Specification Host Rabbit Antigen c Raf Synonyms RAF proto oncogene serine threonine protein kinase; Proto oncogene c RAF (cRaf); Raf 1; RAF; RAF1 Immunogen Synthetic Peptide Location Cytoplasm, Nucleus, Mitochondrion, Cell membrane Accession P04049 Clone Number S 2718 61 Antibody Type Recombinant mAb Isotype IgG Application WB, ICC Reactivity Hu, Ms, Rt, Mk Positive Sample HeLa, A549, C2C12, NIH 3T3, PC 12, COS 7 Purification Protein A
Product Specification
| Host | Rabbit |
| Antigen | c-Raf |
| Synonyms | RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF (cRaf); Raf-1; RAF; RAF1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Mitochondrion, Cell membrane |
| Accession | P04049 |
| Clone Number | S-2718-61 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, A549, C2C12, NIH/3T3, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| ICC | 1:100 | Hu |
Background
c-Raf (also called Raf-1) is a 648-amino-acid serine/threonine kinase that serves as the pivotal upstream cytoplasmic MAP3K in the classical Ras→Raf→MEK→ERK/MAPK cascade: upon growth-factor- or oncogenic Ras-GTP-mediated recruitment to the plasma membrane, it homo- or hetero-dimerizes, becomes phosphorylated on multiple activating residues (Ser338, Tyr341, Thr491, Ser494) while relieving N-terminal autoinhibition, and then processively phosphorylates MEK1/2 to trigger downstream proliferative, anti-apoptotic, and differentiative transcriptional programs; its activity is tightly controlled via feedback and scaffold proteins, and gain-of-function mutations, overexpression, or dysregulated upstream signaling of c-Raf are implicated in numerous cancers, making it a key therapeutic target.
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4.7 ★★★★★
Based on 1360 reviews
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Product Reviews
★★★★★ 4
Walking from La Puy to Finisterre...a well written memoir
Format: Paperback
The key to writing a Camino book--at least from this reader's perspective--it to have a well-written, well-edited, and unique personal account of the adventure. I've read many Camino books that lack these three elements. WTTEOTW has all three elements and is a great addition to anyone's Camino library.
The book is paced nicely and makes for an easy read. I was amused that the author had spent considerable time preparing for this trip yet seemed not to have absorbed some important information prior to the trip--e.g. appropriate footwear, the scramble for nightly accommodations, the frustrations that commonly occur while traveling in unfamiliar cultures. Her adjustments along the way provide humor and insight into preparing for things we've not yet experienced in our own lives.
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Reviewed in the United States on January 8, 2019
★★★★★ 5
really enjoyable
Format: Kindle
I'm thinking of doing the French route myself (starting in Saint-Jean-Pied-de-Port) and have been inhaling other people's stories of the route, whether blog or book form.
The writing is engaging, and both funny and touching in places. I liked that the author is willing to note her own faults as much of those of others', but noted the strengths of herself and everyone around her as well. And her love for her husband jumped off the page, which I really appreciated!
About halfway through the author's time in Spain I found myself on google maps--a surprising portion of the Camino is available on street view!--and yeah, now I *really* want to go. I'm thinking spring of 2023. Fingers crossed.
EDIT: I read the book and wrote this review in late fall of 2021. It is currently March of 2023. I'm flying to France on April 17th, to start in St. Jean Pied de Port. :D
EDIT, number two: The Camino was awesome, and I will probably do it again in a few years, and it was fun to reread this book after doing the Camino and be able to remember so many of the places she talks about. <3
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Reviewed in the United States on December 2, 2021
★★★★★ 5
Well written informative engaging and evocative
Format: Kindle
I've walked to Santiago twice, in 2016 at 65 from St. Jean and in 2019 from Le Puy and bought the book during the pandemic as I waxed nostalgic for the weeks on the road under load with beer bread and bed waiting each afternoon. Beth did a marvelous job of capturing her Camino and much of mine. I can see some of the sights again, the sunrises, the amazing stonework, the clothes drying in the afternoons, the passers by, the cultivated for centuries and still natural landscapes. I almost again feel the foot pain, the rain , the sun, the climbs, the descents, the hot cold on off showers, and the hugs. She helped me again hear the news from Radio Camino, the snoring in the gites and albergues, songs on the road, and the slightly tipsy and slightly loud cafe conversations. I could smell the the morning coffee bowl, the cheeses, the pastries, the animal dropping. And she helped me remember speedy young pilgrims, poor and semi-prosperous middle age Spaniards, paths that were old when followed by Charlemagne, D'Artagnan, and Roman legionnaires, and friends, companions and acquaintancesof all ages and duration. Very well done. Thank you Beth. Buen Camino...
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Reviewed in the United States on July 7, 2020
★★★★★ 5
Entertaining and informative
Format: Kindle
Walking the Camino has been on my bucket list for about 10 years and enjoy reading these types of books. Perhaps because of Justino's background, this is by far the most interesting and entertaining Camino story I've read. For me, it is just the right mix of her daily hiking experience along with a little historical background for context. Her subtle, sometimes self-degrading, humor is a bonus. At the end of the book she provided her daily destinations and miles walked along with a packing list which is something I wish more authors would provide. I'm in my 60's now and beginning to doubt if I will ever make the walk but after reading this book I'm still hoping.
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Reviewed in the United States on April 2, 2019
★★★★★ 4
Camino Memories
Format: Kindle
Well written and organized Caminologue. If you have walked a Camino, this book will bring back fond memories. If you are considering a Camino, this book will inspire you.
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Reviewed in the United States on November 24, 2018
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