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Description
Mouse CRT ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a calreticulin (CRT) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of calreticulin (CRT) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Calreticulin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Calreticulin (CRT), also known as calregulin, CRP55, CaBP3, calsequestrin-like protein, and endoplasmic reticulum-resident protein 60 (ERp60), is a protein encoded by the CALR gene. Calreticulin is a multifunctional soluble protein that binds to Ca2+ ions, inactivating them. It binds with low affinity but has a high capacity, allowing for release in response to signals. Calreticulin is located in storage compartments associated with the endoplasmic reticulum and is considered an ER-resident protein. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
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Product Reviews
★★★★★ 5
Great pillow
Size: King (Pack of 2), Color: Original White
Extremely satisfied. Comfortable. Soft but maintains support of my head and neck. Well made. High quality. Highly recommend.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
★★★★★ 5
Firm for the Win- A pillow that provides support and value!
Size: Standard (Pack of 1), Color: Original White
If you are a firm pillow kind of person... and don't want to spend hundreds on a name brand pillow, then this one is a clear winner! Ideal for back and side sleepers, this pillow seems to adjust to your body over time- becoming more comfortable with more nights spent with it (kind of like a hotel pillow- the first night you're not sure, then you sleep like a baby when you get used to it!).
My husband—an avid memory foam pillow fan—was pleasantly surprised. It took a night or two to adjust, but once he did, this cool pillow’s contours supported his neck beautifully whether he was on his back or side (especially when I nudged him to stop snoring!).
This pillow seems best suited for folks who need a bit more elevation—those with broader shoulders or who prefer higher neck support. It feels solid and delivers the firm support many sleepers crave.
If you lean toward soft, squishy down pillows, this may not be your match. It’s ultra-firm and structured, not plush or compressible. But if firm is your jam, this pillow punches above its price point.
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Reviewed in the United States on November 3, 2025
★★★★★ 3
Nice pillow but now really cooling
Size: King (Pack of 1), Color: Original White
So I got the king size bed not realizing how big it was. So that’s a plus if you’re looking for a large pillow. However, it was too big for me so I will be returning. I also wasn’t impressed with the cooling feature. I have a cooling pillow that truly feels cool and this isn’t it. But the memory foam part of this was excellent. It came out of the packaged and within minutes was fluffy and ready for use. Aside from the size and cooling, I’d recommend this pillow. But I get hot at night so I’d like something more cooling.
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Reviewed in the United States on April 23, 2026
★★★★★ 5
Good to travel fantastic neck support
Size: Camping(Pack of 1), Color: Original White
great confort, unique material, high quality, fantastic neck support
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Reviewed in the United States on May 28, 2026
★★★★★ 1
WHAT A MISTAKE THIS WAS! EXTREMELY SOFT, OFFERING NO SUPPORT AT ALL!
Size: Camping(Pack of 1), Color: Original White, Size: Camping(Pack of 1), Color: Original White
INITIAL Impression: WHAT A MISTAKE THIS WAS! The foam is taking forever to expand, it is a gross color, it is way too soft where it has started to expand. The warranty says it ONLY applies to the original purchaser, which means you cannot give this to anyone as a gift, it would instantly lose its warranty. DO NOT USE ANY EXTERNAL HEAT SOURCE (some memory foam actually suggests putting it in a slightly warm dryer, but to do that would void the warranty.) I got this because I have severe back pain, but it doesn't look like it will ever be firm enough to offer support. It says it will take 24-48 hours to fully expand! I bought it because I need relief NOW, not in 2 days! It has a very limited warranty of only 3 years. The memory foam travel size pillows I bought on Amazon, made by MALOUF, in 2017 came with a 5 year warranty and are still useful, but do need replacing, 9 years later! Unfortunately those pillows are no longer available on Amazon. I chose this small Vaverto memory foam pillow "for camping", because it was available with next morning delivery with some other purchases I made yesterday. (Hoping to be able to use it TODAY.) And because most of the reviews were positive.
It says on the crumpled paper warranty and directions, that ONE side of the pillow is for cooling, but WHICH SIDE? They don't tell you. The order "Shipped at 12:31 AM" the box was cold when it was "Delivered at 9:41 AM". The outdoor temperature had climbed to 51 degrees by then from a low last night of 47 degrees. My room is heated to a comfortable 78 degrees. But we still have a long way to go before this hunk of greenish blue, extremely squishy (I can pinch it at the most expanded area and my fingers nearly touch each other with maybe ¼” of foam separating them. NOT an encouraging sign. There are bits of foam starting to come out of the "punched holes" which appear to be slightly smaller on one side than the other, but WHICH is supposed to be the "cooling side that dissipates heat? Probably the larger holes, but they also weaken the firmness. On closer examination of the crumpled paper that came with it, it says '"TWO-SIDED COVER FOR VERSALITY" Then in a tiny diagram next to that, it says "COOLING SIDE DISSPATES BODY HEAT" with Dissipates spelled wrong! Where is this thing made? Ah.. "CHINA" on a severely crumpled tag inside the cover. It also says the cover is “70% Polyester 30% Viscose Made from Bamboo”. But the Photo shows "Bamboo" in large green letters on the cover along with "VaVerto" in much LARGER grey letters above that. The only letters on my case are the bottom part of a "b" with two "oo" next to it, in a grey-green color, extending from the side seam. NO actual WORDS at all. And the cover looks identical on both sides.
Okay, it's been well over 4 hours in a very warm room, now 80 degrees with the sun pouring in, and it has mostly expanded, but I can still almost touch my fingers together when pinching the thickest part of the pillow, there is still only about ¼” of foam between my fingers. I would describe that as EXTREMELY SOFT. In the actual description of the product, on the Amazon page, if you open the "Features & Specs" it shows "Item Firmness Description - Medium" NOT what the headline says for the product: "Vaverto Small Memory Foam Bed Pillow for Camping - Compressible Medium Firm, Breathable Cover, Machine Washable, Ideal Backpacking".
If I weren’t bedridden in a nursing home this would go back immediately, but it is very hard to get the staff here to help me return ANYTHING that needs to be taken to a store or kiosk. So I may just be out $22.07.
FINAL Impression: WHAT A MISTAKE THIS WAS! EXTREMELY SOFT, OFFERING NO SUPPORT AT ALL!
(PHOTOS ARE NOT ACCURATE to the product!!)
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Reviewed in the United States on May 8, 2026