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Description
Human JAZF1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Juxtaposed With Another Zinc Finger Protein 1 (JAZF1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Juxtaposed With Another Zinc Finger Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Juxtaposed zinc finger protein 1 (JAZF1), also known as TAK1-interacting protein 27 (TIP27) or zinc finger protein 802 (ZNF802), is a protein encoded by the JAZF1 gene. Variants are associated with an increased risk of prostate cancer, type 2 diabetes, and increased height. This gene encodes a nuclear protein with three C2H2-type zinc fingers and functions as a transcriptional repressor. Chromosomal aberrations involving this gene are associated with endometrial stromal tumors. Alternative splice variants encoding different protein isoforms have been described, however, not all variants have been fully characterized. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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Product Reviews
★★★★★ 5
Gentle, yet very effective
Style: Blemish Clearing Toner, Size: 12 Fl Oz (Pack of 1)
I've used multiple exfoliating toners in the past but none were as effective as this one. I have been using it for 3 days now along with the La Roche-Posay Toleriane Purifying Foaming cleanser and it's cleared the whiteheads on my chin quite a bit and made my face feel and look smooth. Will continue to repurchase.
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Reviewed in the United States on May 4, 2026
★★★★★ 5
Finally Something That Works For Me
Style: Blemish Clearing Toner, Size: 12 Fl Oz (Pack of 1)
Imma be honest - I haven’t had great skin for over 30 years. I feel like like there’s always :something: that makes me resort to only being comfortable wearing make-up any day I have to see people / leave the house. But, I started using Thayers in October 2025 (I created my own “Thayers system”: salicylic toner, salicylic pads, and - at times - the stuff in the little dropper bottle) and I can honestly say that :finally: something is working for my skin!
I have tried everything! But Thayers is it for me these days. Thank you!!
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Great product
Size: 25 Count (Pack of 6)
My favorite facial wipes. Gentle to your skin, no alcohol, no burning on the eyes! These wipes clean well make up and don't get dry in the package. Expensive though, hope the price won't go up to make us give up on them.
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Reviewed in the United States on April 5, 2026
★★★★★ 5
The only thing that works for me
Size: 50 count (Pack of 1)
I love these face wipes so much. I cannot use soap due to all the problems I have with my skin. It's so finicky.. I have rosacea with painful P&P and also flare ups of adult acne. I take a med to keep my oil production under control, but that means I end up with a few flakey patches on my cheeks. These are the ONLY face wipes that I've found that not only remove almost all (more on that in a moment) of the makeup I'm wearing, but also seem to help keep my redness under control. I've tried Aveeno Ultra-Calming wipes which profess to help with redness and even those are way too harsh for my skin. The Aveeno wipes make my face sting horrible, leave a greasy residue, and have a smell that is not very pleasant. The Simple wipes take off WAY more makeup than those, or any other wipe, I've tried and leave my skin feeling actually clean and calm.
The only routine that I've found that keeps my skin looking decent is to use these to remove the majority of my makeup and then follow up with Simple Micellar Water
on a cotton pad to remove any further traces. I follow that up with a few spritzes of
(rosewater is supposed to help with redness too) and then moisturize with
- which doesn't have SPF (which makes my rosacea flare up). It sounds like a lot of steps, but it takes me 3 minutes, tops.
I'd just like to add too for those saying that these don't remove waterproof mascara - well, yeah. I would want neither a "gentle" face wipe that was harsh enough to remove waterproof mascara, nor a mascara that was wimpy enough to be removed with a gentle wipe... that would mean it would also come off every time my eyes watered or even just blinked. Just use an oil-free eye makeup remover before using these.
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Reviewed in the United States on March 2, 2016
★★★★★ 5
great clean.
Size: 50 count (Pack of 1)
These get my face clean and exfoliated. I love them.
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Reviewed in the United States on April 2, 2026